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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus
EN
T 219-5
No
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- Organization: KWS SAAT SE & Co. KGaA / PLANTA Applied Plant Genetics and Biotechnology GmbH () | BCH-CON-DE-104960-3Organization:KWS SAAT SE & Co. KGaA / PLANTA Applied Plant Genetics and Biotechnology GmbH ()Private sector (business and industry)Grimsehlstraße 31Einbeck,
37574, GermanyPhone: +49 5561-3110,Fax: +49 5561-311322,Email: info@kws.de,Website: https://www.kws.com, https://www.kws.com/de/de/,
The genetically modified sugarbeets were modified to constitutively express the coat protein gene of the Beet Necrotic Yellow Vein Virus (BNYVV), an important domain for encapsidation, vector transmissibility and cell-to-cell transmission.
The constitutively expressed capsid protein gene gives rise to a concentration of coat protein in every cell of the genetically modified plant tissue which is far below the concentration found in plants infected with BNYVV. The gus gene was inserted for the histological determination of a successful transformation.
As a result of the genetic modification a BNYVV resistance of the modified plants is expected.
EN
The constitutively expressed capsid protein gene gives rise to a concentration of coat protein in every cell of the genetically modified plant tissue which is far below the concentration found in plants infected with BNYVV. The gus gene was inserted for the histological determination of a successful transformation.
As a result of the genetic modification a BNYVV resistance of the modified plants is expected.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12097-4 Organism Beta vulgaris (Common beet, Sugarbeet, BETMA)Crops
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Sugarbeet modified for resistance to the Beet Necrotic Yellow Vein Virus| PLANTA Angewandte Pflanzengenetik und Biotechnologie GmbH | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Viruses, Beet necrotic yellow virus (BNYV))
Derivative of pBI121
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-110730-1 BNYVV coat protein | Beet necrotic yellow vein virus (BNYVV)Protein coding sequence | Resistance to diseases and pests (Viruses, Beet necrotic yellow virus (BNYV))
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-110731-2 Light inducible tissue-specific LS1 gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | Selectable marker genes and reporter genes
The cDNA of the coat protein gene of the Beet Necrotic Yellow Vein Virus (BNYVV) is expressed under the control of the double 35S promoter of the Cauliflower mosaic virus (CaMV). The CaMV 35S-terminator is used as a termination signal.
Additionally, the gus-gene from Escherichai coli, in which an intron of the ST-LS1 gene from Solanum tuberosum was inserted, is expressed under the control of the CaMV 35S promoter. The terminator region of the nopaline synthase gene (nos) from A. tumefaciens is used as a termination signal.
The neomycin phosphotransferase gene (nptII), expressed under the control of the promoter and termination signal of the nopaline synthase gene (nos) of Agrobacterium tumefaciens, is used as a selection marker.
The introduced nucleic acid is integrated in the genome of the recipient organism at 7 - 9 insertion sites.
EN
Additionally, the gus-gene from Escherichai coli, in which an intron of the ST-LS1 gene from Solanum tuberosum was inserted, is expressed under the control of the CaMV 35S promoter. The terminator region of the nopaline synthase gene (nos) from A. tumefaciens is used as a termination signal.
The neomycin phosphotransferase gene (nptII), expressed under the control of the promoter and termination signal of the nopaline synthase gene (nos) of Agrobacterium tumefaciens, is used as a selection marker.
The introduced nucleic acid is integrated in the genome of the recipient organism at 7 - 9 insertion sites.
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- Research
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