Living Modified Organism (LMO) Registry

Record ID	Unique Identification	Identity & Transformation Event	Organism	Description
14753	ACS-BNØØ1-4	InVigor™ Canola RF1 (B93-101)	Brassica napus - Rapeseed, Canola plant, Canola	Canola fertility restoration system displaying glufosinate herbicide tolerance. Contains the barstar gene from Bacillus amyloliquefaciens, and the bar gene from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium). Also contains neomycin phosphotransferase II (npt II) conferring kanamycin resistance.
14754	ACS-BNØØ2-5	InVigor™ Canola RF2 (B94-2)	Brassica napus - Rapeseed, Canola plant, Canola	Canola fertility restoration system displaying glufosinate herbicide tolerance. Contains the barstar gene from Bacillus amyloliquefaciens, and the bar gene from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium). Also contains neomycin phosphotransferase II (npt II) conferring kanamycin resistance.
14755	ACS-BNØØ3-6	InVigor™ Canola RF3	Brassica napus - Rapeseed, Canola plant, Canola	Canola fertility restoration system displaying glufosinate herbicide tolerance. Contains the barstar gene from Bacillus amyloliquefaciens, and the bar gene encoding phosphinothricin N-acetyltransferase (PAT) from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).
14756	ACS-BNØØ4-7	InVigor™ Canola MS1 (B91-4)	Brassica napus - Rapeseed, Canola plant, Canola	Canola male-sterility system displaying glufosinate herbicide tolerance. Contains the barnase gene from Bacillus amyloliquefaciens and the bar gene encoding phosphinothricin N-acetyltransferase from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium). Also contains the neomycin phosphotransferase II (npt II) gene conferring resistance to the antibiotic kanamycin.
14757	ACS-BNØØ4-7 x ACS-BNØØ1-4	MS1 x RF1 PGS1 (MS1(B91-4) x RF1(B93-101))	Brassica napus - Rapeseed, Canola plant, Canola	Canola with male-sterility, fertility restoration, pollination control system, and glufosinate herbicide tolerance. MS1 line contained the barnase gene from Bacillus amyloliquefaciens (with pTa 29 pollen specific promoter from Nicotiana tabacum). RF1 line contained the barstar gene from the same bacteria with anther-specific promoter, and both lines contained the bar gene encoding phosphinothricin N-acetyltransferase (PAT) from Streptomyces hygroscopicus (with PSsuAra promoter from Arabidopsis thaliana) to confer tolerance to the herbicide glufosinate. Also includes neomycin phosphotransferase II (npt II) gene (with nopaline synthase -nos) promoter from A. tumefaciens) conferring resistance to the antibiotic kanamycin.
14758	ACS-BNØØ4-7 x ACS-BNØØ2-5	PGS2 (MS1 x RF2) (B91-4 x B94-2)	Brassica napus - Rapeseed, Canola plant, Canola	Canola with male-sterility, fertility restoration, pollination control system displaying glufosinate herbicide tolerance. MS1 line contained the barnase gene from Bacillus amyloliquefaciens (with pTa 29 pollen specific promoter from Nicotiana tabacum). RF2 line contained the barstar gene from the same bacteria with anther-specific promoter. Both lines contained the bar gene encoding phosphinothricin N-acetyltransferase (PAT) from Streptomyces hygroscopicus (with PSsuAra promoter from Arabidopsis thaliana) to confer tolerance to the herbicide glufosinate. Also both lines include the neo II (neomycin phosphotransferase II or nptII) gene with nopaline synthase (nos) promoter from A. tumefaciens) conferring resistance to the antibiotic kanamycin.
14759	ACS-BNØØ5-8	InVigor™ Canola MS8	Brassica napus - Rapeseed, Canola plant, Canola	Canola with male-sterility system displaying glufosinate herbicide tolerance. Contains the barnase gene from Bacillus amyloliquefaciens and the bar gene encoding phosphinothricin N-acetyltransferase from Streptomyces hygroscopicus. Also contains the neomycin phosphotransferase II (npt II) gene conferring resistance to the antibiotic kanamycin.
14760	ACS-BNØØ5-8 x ACS-BNØØ3-6	InVigor™ Canola MS8 x RF3	Brassica napus - Rapeseed, Canola plant, Canola	Canola with male-sterility, fertility restoration, pollination control system displaying glufosinate herbicide tolerance. MS8 line contained a copy of the barnase gene from Bacillus amyloliquefaciens. The barnase gene was regulated by the pTa29 anther specific promoter from Nicotiana tabacum and terminated by part of the 3´non-coding region - 3´nos - of the nopaline synthase gene of A. tumefaciens. RF3 line contained the barstar gene (barnase ribonuclease inhibitor) from Bacillus amyloliquefaciens and was regulated by the pTa29 anther-specific promoter from N. tabacum and the nos termination signal. Both lines contained the bar gene from Streptomyces hygroscopicus, encoding phosphinothricin N-acetyltransferase (PAT). Expression of the bar gene was regulated by the PSsuAra promoter from Arabidopsis thaliana and post-translational targeting of the gene product to the chloroplast organelles was accomplished by fusion of the 5'-terminal coding sequence with the chloroplast transit peptide DNA sequence from A. thaliana. There were no marker genes for antibiotic resistance present in the transformed plants.
14761	ACS-BNØØ7-1	Liberty-Link™ Innovator Canola Topas 19/2 (HCN92)	Brassica napus - Rapeseed, Canola plant, Canola	Glyphosate herbicide tolerant canola produced by inserting the phosphinothricin acetyltransferase (pat) gene conferring tolerance to Phosphinothricin (Glufosinate ammonium) herbicide and neomycin phosphotransferase II (npt II) conferring resistance to the herbicide kanamycin.
14762	ACS-BNØØ8-2	InVigor™ Canola T45 (HCN28)	Brassica napus - Rapeseed, Canola plant, Canola	Glufosinate tolerant canola with insertion of the phosphinothricin acetyltransferase (pat) gene from Streptomyces viridochromogenes, conferring tolerance to phosphinothricin (Glufosinate ammonium) herbicide.
46388	ACS-BNØØ9-3	Liberator™ Rapeseed pHoe6/Ac pHoe6/Ac	Brassica napus - Rapeseed, Canola plant, Canola	Canola with tolerance to the herbicide phosphinothricin (Glufosinate ammonium) conferred through insertion of a synthetic copy of the phosphinothricin acetyltransferase (pat) gene from the aerobic actinomycete Streptomyces viridochromogenes. In addition to the synthetic pat gene, Liberator™ Rapeseed (pHoe6/Ac) has the following exogenous DNA inserts: (a) Right border sequence from Ti-plasmid pTiT37, necessary for DNA transfer (25 bp) from Agrobacterium tumefaciensT37 (Nopalin strain) (b) T-DNA partial sequence from Ti plasmid pTiT37, no function in plants (184 bp) from Agrobacterium tumefaciens T37 (Nopalin strain) (c) Polylinker sequence from plasmid vector pUC19 (51 bp) of synthetic origin (d) Promoter from the 35S-RNA (530 bp) from Cauliflower Mosaic Virus (CMV) (e) Terminator of the 35S-RNA (207 bp) from Cauliflower Mosaic Virus (CMV) (f) T-DNA partial sequence of the Ti plasmid pTiAch5, with no known function in plants (277 bp) from Agrobacterium tumefaciens Ach5 (Octopin strain) (g) Left border sequence from Ti plasmid pTiAch5, necessary for DNA transfer (24 bp) from Agrobacterium tumefaciens Ach5 (Octopin strain)
15101	ACS-BNØ1Ø-4	Falcon™ Rapeseed GS40/90pHoe6/Ac GS40/90pHoe6/Ac	Brassica napus - Rapeseed, Canola plant, Canola	Canola with tolerance to the herbicide phosphinothricin (Glufosinate ammonium) conferred through insertion of a synthetic copy of the phosphinothricin acetyltransferase (pat) gene from the aerobic actinomycete Streptomyces viridochromogenes. In addition to the synthetic pat gene, Liberator™ Rapeseed (pHoe6/Ac) has the following exogenous DNA inserts: (a) Right border sequence from Ti-plasmid pTiT37, necessary for DNA transfer (25 bp) from Agrobacterium tumefaciensT37 (Nopalin strain) (b) T-DNA partial sequence from Ti plasmid pTiT37, no function in plants (184 bp) from Agrobacterium tumefaciens T37 (Nopalin strain) (c) Polylinker sequence from plasmid vector pUC19 (51 bp) of synthetic origin (d) Promoter from the 35S-RNA (530 bp) from Cauliflower Mosaic Virus (CMV) (e) Terminator of the 35S-RNA (207 bp) from Cauliflower Mosaic Virus (CMV) (f) T-DNA partial sequence of the Ti plasmid pTiAch5, with no known function in plants (277 bp) from Agrobacterium tumefaciens Ach5 (Octopin strain) (g) Left border sequence from Ti plasmid pTiAch5, necessary for DNA transfer (24 bp) from Agrobacterium tumefaciens Ach5 (Octopin strain)
14752	ACS-BNØ11-5	Navigator™ Canola Oxy-235	Brassica napus - Rapeseed, Canola plant, Canola	Canola (veriety Westar) tolerant to the oxynil herbicides created through insertion of the bxn gene isolated from the bacterium Klebsiella ozaenae, encoding a nitrilase enzyme that hydrolyzes oxynil herbicides to non-phytotoxic compounds.
14763	ACS-BVØØ1-3	Herbicide-tolerant Sugar Beet T120-7	Beta vulgaris - Sugarbeet	Sugar beet tolerant to the herbicide glufosinate, created through introduction of the phosphinothricin acetyltransferase (pat) gene from Streptomyces viridochromogenes, an aerobic soil bacteria, which confers tolerance to the herbicide Phosphinothricin (Glufosinate ammonium). Neomycin phosphotransferase II (npt II) confers tolerance to the antibiotic kanamycin.
14851	ACS-GHØØ1-3	Liberty Link™ Cotton LLCotton25	Gossypium - Cotton	Cotton tolerant to glufosinate ammonium herbicide produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (bar) from the soil bacterium Streptomyces hygroscopicus.
30886	ACS-GHØØ1-3 x MON-15985-7	Liberty Link™ Bollgard II™ Cotton LLCotton25 x MON15985	Gossypium - Cotton	A stacked insect-resistant and herbicide-tolerant cotton derived from conventional cross-breeding of ACS-GHØØ1-3 with MON-15985-7. Tolerance to the herbicide glufosinate ammonium was produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (bar) from Streptomyces hygroscopicus, and resistance to lepidoptera insects is conferred from the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis. Product of traditional plant breeding...
14852	ACS-GMØØ1-8	Herbicide-tolerant Soybean W98	Glycine max - Soybeans	Soybean tolerant to glufosinate ammonium herbicide produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (pat) from the soil bacterium Streptomyces hygroscopicus.
14853	ACS-GMØØ2-9	Herbicide-tolerant Soybean W62	Glycine max - Soybeans	Soybean tolerant to glufosinate ammonium herbicide produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (pat) from the soil bacterium Streptomyces hygroscopicus.
14854	ACS-GMØØ3-1	Herbicide-tolerant Soybean GU262	Glycine max - Soybeans	Glufosinate ammonium herbicide tolerant soybean produced by inserting a modified phosphinothricin acetyltransferase (PAT) encoding gene (pat) from the soil bacterium Streptomyces viridochromogenes.
14855	ACS-GMØØ4-2	Herbicide-tolerant Soybean A2704-21	Glycine max - Soybeans	Phosphinothricin (Glufosinate ammonium) herbicide tolerant soybean produced by inserting a modified phosphinothricin acetyltransferase (pat) gene from the soil bacterium Streptomyces viridochromogenes.
14764	ACS-GMØØ5-3	Herbicide-tolerant Soybean A2704-12	Glycine max - Soybeans	Phosphinothricin (Glufosinate ammonium) herbicide tolerant soybean produced by inserting a modified phosphinothricin acetyltransferase (pat) gene from the soil bacterium Streptomyces viridochromogenes.
14857	ACS-GMØØ6-4	Liberty Link™ Soybean A5547-127	Glycine max - Soybeans	Phosphinothricin (Glufosinate ammonium) herbicide tolerant soybean produced by inserting a modified phosphinothricin acetyltransferase (pat) gene from the soil bacterium Streptomyces viridochromogenes.
14858	ACS-OSØØ1-4	Liberty Link™ Rice LLRICE06	Oryza sativa - Rice	Rice resistant to the herbicide glufosinate through introduction of the bar gene from Streptomyces hygroscopicus which encodes the enzyme phosphinothricin-N-acetyltransferase (PAT) that catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound.
14859	ACS-OSØØ2-5	Liberty Link™ Rice LLRICE62	Oryza sativa - Rice	Rice resistant to the herbicide glufosinate through introduction of the bar gene from Streptomyces hygroscopicus which encodes the enzyme phosphinothricin-N-acetyltransferase (PAT) that catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound.
14765	ACS-ZMØØ1-9	Herbicide-tolerant Maize MS3	Zea mays - Maize, Corn	Male sterility in maize caused by expression of the barnase ribonuclease gene from Bacillus amyloliquefaciens and phosphinothricin acetyltransferase (bar) gene conferring tolerance to the herbicide glufosinate.
14766	ACS-ZMØØ2-1	Liberty Link™ Maize T14	Zea mays - Maize, Corn	Maize with tolerance to the herbicide phosphinothricin (Glufosinate ammonium) conferred through insertion of the phosphinothricin acetyltransferase (pat) gene from the aerobic actinomycete Streptomyces viridochromogenes.
14767	ACS-ZMØØ3-2	Liberty Link™ Maize T25	Zea mays - Maize, Corn	Maize with tolerance to the herbicide phosphinothricin (Glufosinate ammonium) conferred through insertion of the phosphinothricin acetyltransferase (pat) gene from the aerobic actinomycete Streptomyces viridochromogenes.
15373	ACS-ZMØØ3-2 x MON-ØØ81Ø-6	Liberty Link™ Yieldgard™ Maize T25 x MON810	Zea mays - Maize, Corn	A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of ACS-ZMØØ3-2 with MON-ØØ81Ø-6. Tolerance to the herbicide phosphinothricin (Glufosinate ammonium) is conferred through insertion of the phosphinothricin acetyltransferase (pat) gene from the aerobic actinomycete Streptomyces viridochromogenes. Insect-resistance is produced by inserting a truncated form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki HD-1 which affords resistance to attack by the European corn borer (ECB), Ostrinia nubilalis.
14862	ACS-ZMØØ4-3	Starlink™ Maize CBH-351	Zea mays - Maize, Corn	Maize with insect resistance and herbicide tolerance through introduction of the cry9C gene from Bacillus thuringiensis and the bar gene from Streptomyces hygroscopicus.
14863	ACS-ZMØØ5-4	InVigor™ Maize MS6	Zea mays - Maize, Corn	Maize male-sterility system displaying glufosinate herbicide tolerance. Contains the barnase gene from Bacillus amyloliquefaciens and the bar gene encoding phosphinothricin N-acetyltransferase from Streptomyces hygroscopicus.
46334	BCS-GHØØ2-5	GlyTolTM cotton GHB614	Gossypium hirsutum - Cotton	GlyTol cotton event GHB614 contains a stably integrated gene 2mepsps, which encodes the 2mEPSPS protein. The gene was introduced by Agrobacterium-mediated gene transfer. Southern blot analyses show that this transformation event contains one complete copy of the 2mepsps gene. The 2mepsps gene was generated by introducing mutations into the wild-type epsps (wt epsps) gene from maize, leading to a double mutant EPSPS protein with two amino acid substitutions (2mEPSPS). This modification confers the protein a decreased binding affinity for glyphosate, allowing it to maintain sufficient enzymatic activity in the presence of the herbicide. Therefore, the plants bearing this gene are tolerant to glyphosate herbicides.
15100	BPS-25271-9	Modified starch Potato EH92-527-1	Solanum tuberosum - Potato	Potato containing starch with increased amylopectin content through introduction of the gbss gene in antisense direction, which inactivates native gene, causing the production of starch with little or no amylose. The nptII gene confers resistance to the antibiotic kanamycin.
14941	BXN-1Ø211-9	BXN™ Cotton BXN10211 (10211)	Gossypium - Cotton	Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The aphII gene was isolated from the bacterium Eschericia coli and confers tolerance to the antibiotic kanamycin (used as a selectable marker).
14823	BXN-1Ø215-4	BXN™ Cotton BXN10215 (10215)	Gossypium - Cotton	Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The aphII gene was isolated from the bacterium Eschericia coli confers tolerance to the antibiotic kanamycin (used as a selectable marker).
14825	BXN-1Ø222-2	BXN™ Cotton BXN10222 (10222)	Gossypium - Cotton	Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The kanamycin-resistance gene aphII was isolated from the bacterium Eschericia coli.
14824	BXN-1Ø224-4	BXN™ Cotton BXN10224 (10224)	Gossypium - Cotton	Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The kanamycin-resistance gene aphII was isolated from the bacterium Eschericia coli.
14768	CDC-FLØØ1-2	CDC Triffid (Flax / Linseed) FP967	Linum usitatissimum - Flax, Linseed	Linseed tolerant to the herbicide sufonylurea through insertion of the acetolactate synthase (als) gene. Neomycin phosphotransferase II (neo) confers resistance to the antibiotic kanamycin and the nos gene codes for nopaline synthase; these were used as selectable markers.
14780	CGN-89111-8	High oleic acid Canola 23-18-17 (Event 18) (pCGN3828-212/86-18)	Brassica napus - Rapeseed, Canola plant, Canola	High laurate and myristate canola produced by inserting a thioesterase (te) encoding gene from the California bay laurel (Umbellularia californica). The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.
15425	CGN-89322-3	Delayed-ripening Tomatoes 8338	Solanum lycopersicum - Tomato	Delayed ripening tomatoes, created through Introduction of a gene sequence encoding the enzyme 1-amino-cyclopropane-1-carboxylic acid deaminase (ACCd) that metabolizes the precursor of the fruit ripening hormone ethylene. The neomycin phosphotransferase II (npt II) confers resistance to the antibiotic kanamycin.
14781	CGN-89322-3	Delayed-ripening Tomatoes 8338	Solanum lycopersicum - Tomato	Delayed ripening tomatoes, created through Introduction of a gene sequence encoding the enzyme 1-amino-cyclopropane-1-carboxylic acid deaminase (ACCd) that metabolizes the precursor of the fruit ripening hormone ethylene. The neomycin phosphotransferase II (npt II) confers resistance to the antibiotic kanamycin.
14782	CGN-89465-2	High oleic acid Canola 23-198 (Event 23) (pCGN3828-212/86-23)	Brassica napus - Rapeseed, Canola plant, Canola	Canola with high oleic acid oil content (laurate and myristate) produced by inserting a thioesterase encoding gene from the California bay laurel (Umbellularia californica). The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.
14867	CGN-89564-2	FLAVR SAVR™ Tomato FLAVR-SAVR (N73 1436-111, CR3-613, CR3-623)	Solanum lycopersicum - Tomato	Tomatoes with delayed softening from suppression of polygalacturonase (PG) enzyme activity through inclusion of an "antisense" polygalacturonase gene from tomatoes. The nptII gene confers resistance to the antibiotic kanamycin and was used a marker gene.
40294	CUH-CP551-8	Papaya resistant to viral infection (papaya ringspot virus - PRSV) 55-1	Carica papaya - Papaya	The transgenic papaya line 55-1 was produced by biolistic (particle bombardment) transformation of embryogenic cultures of the papaya cultivar ‘Sunset’ with DNA-coated tungsten particles. The Agrobacterium tumefaciens binary plasmid pGA482GG/cpPRSV-4 used for the transformation contained three plant-expressible genes, the PRSV CP, neo, and uidA genes. The plasmid also had two genes encoding resistance to tetracycline and gentamycin antibiotics, respectively, but their associated DNA regulatory sequences enabled expression only in bacteria. The plasmid included the right- and left-border regions derived from the A. tumefaciens T-DNA. Expression of the PRSV CP gene was controlled by including promoter and transcription termination and polyadenylation signal sequences derived from the 35S transcript of cauliflower mosaic virus (CaMV). In addition, the CP gene sequences were fused to the 5' untranslated sequence and the first 39 nucleotides from the cucumber mosaic virus (CMV) CP to enhance translation of the transgene mRNA. The inclusion of these additional sequences was necessary because PRSV naturally encodes its CP as part of a polyprotein and, therefore, the CP coding region normally lacks a translation initiation ATG codon. Expression of the neo gene was under control of the promoter and terminator sequences from the nopaline synthase (nos) gene of A. tumefaciens. The second marker gene, uidA, was modified for plant expression by the addition of 35S promoter region from CaMV and the nos 3'-termination region. Southern blot analyses of genomic DNA from line 55-1 verified that it contained the PRSV CP gene, intact copies of two plant-expressible marker genes encoding NPTII and GUS, respectively, and a partial copy of the tetracycline resistance marker gene. Genomic DNA did not hybridize with probes to the gentamycin marker genes or to the origin of bacterial replication (Ori V/Tet) region. The partial tetracycline resistance gene was not expressed in plants, due both to the fact that the gene was incomplete and under the regulatory control of a bacterial promoter.
40296	CUH-CP631-7	Papaya resistant to viral infection (papaya ringspot virus - PRSV) 63-1	Carica papaya - Papaya	The transgenic papaya line 63-1 was produced by biolistic (particle bombardment) transformation of embryogenic cultures of the papaya cultivar ‘Sunset’ with DNA-coated tungsten particles. The Agrobacterium tumefaciens binary plasmid pGA482GG/cpPRSV-4 used for the transformation contained three plant-expressible genes, the PRSV CP, neo, and uidA genes. The plasmid also had two genes encoding resistance to tetracycline and gentamycin antibiotics, respectively, but their associated DNA regulatory sequences enabled expression only in bacteria. The plasmid included the right- and left-border regions derived from the A. tumefaciens T-DNA. Expression of the PRSV CP gene was controlled by including promoter and transcription termination and polyadenylation signal sequences derived from the 35S transcript of cauliflower mosaic virus (CaMV). In addition, the CP gene sequences were fused to the 5' untranslated sequence and the first 39 nucleotides from the cucumber mosaic virus (CMV) CP to enhance translation of the transgene mRNA. The inclusion of these additional sequences was necessary because PRSV naturally encodes its CP as part of a polyprotein and, therefore, the CP coding region normally lacks a translation initiation ATG codon. Expression of the neo gene was under control of the promoter and terminator sequences from the nopaline synthase (nos) gene of A. tumefaciens. The second marker gene, uidA, was modified for plant expression by the addition of 35S promoter region from CaMV and the nos 3'-termination region. Southern blot analyses indicated that line 63-1 contained intact, functional genes encoding the PRSV CP and NPTII, and did not contain the GUS encoding gene. Genomic hybridization with probes to the gentamycin resistance gene and the Ori T/Tet region indicated that either all or part of the genes for gentamycin and tetracycline resistance had integrated into the papaya genome. However, these genes were not functional since their bacterial promoters cannot drive expression of these genes in plants.
14841	DAS-Ø15Ø7-1	Herculex™ I Maize TC1507	Zea mays - Maize, Corn	Insect-resistant and glufosinate ammonium herbicide tolerant maize produced by inserting the cry1F gene from Bacillus thuringiensis var. aizawai which confers resistance against certain lepidopteran pests, such as the European corn borer (Ostrinia nubilalis) and Sesamia spp, and the phosphinothricin N-acetyltransferase encoding gene (PAT) from Streptomyces viridochromogenes which confers tolerance to application of glufosinate-ammonium herbicide.
15186	DAS-Ø15Ø7-1 x DAS-59122-7	Herculex XTRA™ Conventional cross breeding between LMOs TC1507 and DAS-59122	Zea mays - Maize, Corn	A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of DAS-Ø15Ø7-1 with DAS-59122-7. Insect resistance is conferred by the cryIF, cry34Ab1 and cry35Ab1 genes from the common soil bacterium Bacillus thuringiensis, and glufosinate herbicide tolerance through the pat gene from Streptomyces viridochromogenes.
15185	DAS-Ø15Ø7-1 x DAS-59122-7 x MON-ØØ6Ø3-6	Herculex XTRA™ x NK603 TC1507 x DAS-59122 x NK603	Zea mays - Maize, Corn	A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of DAS-59122-7 x DAS-Ø15Ø7-1 and MON-ØØ6Ø3-6. Insect resistance is conferred by the cry34Ab1, cry35Ab1 and cryIF genes from the common soil bacterium Bacillus thuringiensis, and glyphosate herbicide tolerance through the cp4 epsps gene from Agrobacterium ssp. strain CP4 and glufosinate-ammonium herbicide tolerance through the pat gene from Streptomyces viridochromogenes.
14808	DAS-Ø15Ø7-1 X MON-ØØ6Ø3-6	Herculex® I Insect Protection with Roundup Ready® 2 (1507 x NK603) Conventional cross breeding between LMOs 1507 and NK603	Zea mays - Maize, Corn	A stacked lepidopteran insect-resistant and herbicide-tolerant (glufosinate and glyphosate) maize, through incorporation of the cryIF gene from Bacillus thuringiensis var. aizawai, cp4 epsps gene from Agrobacterium ssp. strain CP4 and the pat gene from Streptomyces viridochromogenes.
15187	DAS-Ø6275-8	TC6275 Maize TC6275	Zea mays - Maize, Corn	Insect-resistant and glufosinate ammonium herbicide tolerant maize produced by inserting the cry1F gene from Bacillus thuringiensis var. aizawai which confers resistance against certain lepidopteran pests, such as the European corn borer (Ostrinia nubilalis) and Sesamia spp, and the phosphinothricin N-acetyltransferase encoding gene (PAT) bar gene from Streptomyces hygroscopicus which confers tolerance to application of glufosinate-ammonium herbicide.
14938	DAS-21Ø23-5	Insect-resistant Cotton 3006-210-23	Gossypium - Cotton	Lepidopteran-resistant cotton, with insect resistance conferred by the cry1Ac gene from the common soil bacterium Bacillus thuringiensis (Bt) var. kurstaki.
14798	DAS-21Ø23-5 x DAS-24236-5 x MON-Ø1445-2	WideStrike™ Roundup Ready™ Cotton 3006-210-23 x 281-24-236 x MON1445	Gossypium - Cotton	A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of DAS-21Ø23-5 x DAS-24236-5 with MON-Ø1445-2. Inserted genes include the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki, the cry1F gene from Bacillus thuringiensis var. aizawai, the PAT encoding gene from Streptomyces viridochromogenes, and a naturally glyphosate tolerant form of the enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens strain CP4 to confer tolerance to phosphinothricin (glyphosate) herbicide.
15169	DAS-21Ø23-5 x DAS-24236-5 x MON-88913-8	Widestrike x Roundup Ready Flex™ Cotton 3006-210-23 x 281-24-236 x MON88913	Gossypium - Cotton	A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of DAS-21Ø23-5 x DAS-24236-5 with MON-88913-8. Inserted genes include the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki, the cry1F gene from Bacillus thuringiensis var. aizawai, the PAT encoding gene from Streptomyces viridochromogenes, and a naturally glyphosate tolerant form of the enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens strain CP4 to confer tolerance to phosphinothricin (glyphosate) herbicide.
14940	DAS-24236-5	Insect-resistant Cotton 281-24-236	Gossypium - Cotton	Lepidopteran-resistant cotton, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai.
15107	DAS-24236-5 x DAS-21Ø23-5	WideStrike™ insect-resistant Cotton 281-24-236 x 3006-210-23	Gossypium - Cotton	A stacked lepidopteran insect-resistant cotton derived from conventional cross-breeding of DAS-21Ø23-5 and DAS-24236-5, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis var. aizawai and the cry1Ac gene from B. thuringiensis var. kurstaki.
15165	DAS-59122-7	Herculex® RW Rootworm Protection Maize 59122	Zea mays - Maize, Corn	Coleopteran-resistant and herbicide-tolerant maize, with insect resistance conferred by the cry34Ab1 and cry35Ab1 genes from the common soil bacterium Bacillus thuringiensis and herbicide tolerance conferred by the pat gene from Streptomyces viridochromogenes.
15178	DAS-59122-7 x MON-ØØ6Ø3-6	Herculex® RW Rootworm Protection with Roundup Ready® 2 Maize Conventional cross breeding between LMOs 59122 and NK603	Zea mays - Maize, Corn	A stacked insect-resistant, glyphosate and glufosinate ammonium-tolerant maize derived from conventional cross-breeding of DAS-59122-7 with MON-ØØ6Ø3-6. Inserted genes include the Bt toxin-producing cry34Ab1 and cry35Ab1 genes from the common soil bacterium Bacillus thuringiensis, and herbicide tolerance conferred by the pat gene from Streptomyces viridochromogenes and the cp4 epsps gene encoding 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) from Agrobacterium tumefaciens.
14783	DD-Ø1951A-7	Herbicide-tolerant Cotton 19-51a	Gossypium - Cotton	Cotton tolerant to the sulfonylurea herbicide through introduction of a variant form of acetolactate synthase (ALS) from the chimeric gene S4-HrA originally derived from two different tobacco (Nicotiana tabacum cv. Xanthi) ALS genes that both encoded herbicide sensitive versions of ALS.
14769	DD-Ø26ØØ5-3	High oleic acid Soybean 260-05 (G94-1, G94-19, G168)	Glycine max - Soybeans	High oleic acid soybean produced by inserting a second copy of the fatty acid desaturase (GmFad2-1) gene from soybean, which resulted in "silencing" of the endogenous host gene.
14770	DKB-89614-9	Bt Xtra™ Insect-resistant Maize DBT418	Zea mays - Maize, Corn	Insect-resistant and glufosinate ammonium herbicide tolerant maize developed by inserting the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki and the phosphinothricin acetyltransferase (bar) gene from Streptomyces hygroscopicus.
14771	DKB-8979Ø-5	Glufosinate-tolerant Maize DLL25 (B16)	Zea mays - Maize, Corn	Glufosinate ammonium herbicide tolerant maize produced by inserting the phosphinothricin acetyltransferase (bar) gene from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).
14840	FLO-ØØØØ4-9	Colour-modified carnation 4	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida. A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14837	FLO-ØØØ15-2	Colour-modified carnation 15	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida. A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14838	FLO-ØØØ16-3	Colour-modified carnation 16	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida. A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14839	FLO-ØØØ66-8	Long vase life carnation 66	Dianthus caryophyllus - Carnations	Long vase life (delayed senescence) carnations produced by inserting a truncated copy of the carnation 1-amino-cyclopropane-1-carboxylic acid (ACC) synthase encoding gene in order to suppress expression of the endogenous unmodified gene, which is required for normal ethylene biosynthesis. Tolerance to sulfonyl urea herbicides was via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco.
14836	FLO-Ø7442-4	Moondust™ Carnation 11 (7442)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida. A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14829	FLO-11226-8	Colour-modified carnation 1226A (11226)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the bp40 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H). A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14830	FLO-11351-7	Colour-modified carnation 1351A (11351)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the bp40 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H). A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14772	FLO-11363-1	Moonshadow™ carnation 11363 (1363A)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the bp40 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H). A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14833	FLO-114ØØ-2	Colour-modified carnation 1400A (11400)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the bp40 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H). A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14831	FLO-11959-3	Colour-modified carnation 959A (11959)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the bp40 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H). A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14832	FLO-11988-7	Colour-modified carnation 988A (11988)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the bp40 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H). A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14828	FLO-4Ø619-7	Moonshade™ Carnation 123.2.2 (40619)	Dianthus caryophyllus - Carnations	Carnations with modified colour and sulfonylurea herbicide tolerance, produced by inserting two anthocyanin biosynthetic genes from Petunia hybrida, dihydroflavonol reductase (dfr) and Hf1 encoding flavonoid 3',5'hydroxylase (F3'5'H), whose expression results in a violet/mauve colouration. Tolerance to sulfonyl urea herbicides was produced through the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from Nicotiana tabacum.
14834	FLO-4Ø644-4	Moonlite™ Carnation 123.2.38 (40644)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida. A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
14835	FLO-4Ø685-1	Moonvista™ Carnation 123.8.8 (40685)	Dianthus caryophyllus - Carnations	Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the Hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida. A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.
43797	IFD-524Ø1-4	WKS82/130-4-1	Rosa x hybrida L. - Rose	This rose line contains an introduced gene encoding flavonoid 3’, 5’-hydroxylase (F3’5’H) enzyme from Viola x wittrockiana involved in the synthesis of delphinidin and a gene encoding anthocyanin 5-acyltransferase from torenia (Torenia hybrida), which modifies the delphinidin produced.
43796	IFD-529Ø1-9	WKS82/130-9-1	Rosa x hybrida L. - Rose, Torenia hybrida - Torenia	This rose line contains an introduced gene encoding flavonoid 3’, 5’-hydroxylase (F3’5’H) enzyme from Viola x wittrockiana involved in the synthesis of delphinidin and a gene encoding anthocyanin 5-acyltransferase from torenia (Torenia hybrida), which modifies the delphinidin produced.
14773	KM-ØØØH71-4	Roundup Ready™ Sugar Beet H7-1	Beta vulgaris - Sugarbeet	Glyphosate herbicide tolerant sugar beet produced by inserting the gene encoding the enzyme 5-enolypyruvylshikimate-3-phosphate synthase (epsps) from the CP4 strain of Agrobacterium tumefaciens.
14794	MON-ØØØ21-9	Roundup Ready™ Maize GA21 (G21)	Zea mays - Maize, Corn	Glyphosate tolerant maize created through introduction of a modified gene encoding 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS), an enzyme involved in the shikimate biochemical pathway for the production of the aromatic amino acids. The bla gene confers tolerance to the antibiotic ampicillin and was used as a selectable marker.
14892	MON-ØØØ21-9 x MON-ØØ81Ø-6	Roundup Ready™ YieldGard™ Maize GA21 x MON810	Zea mays - Maize, Corn	A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of MON-ØØØ21-9 and MON-ØØ81Ø-6. Tolerance to the herbicide glyphosate produced through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (epsps), that confers tolerance to the herbicide glyphosate and resistance to lepidoptera insects from the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki.
14795	MON-ØØØ73-7	Roundup Ready™ Canola RT73 (GT73)	Brassica napus - Rapeseed, Canola plant, Canola	Glyphosate herbicide tolerant canola (Westar variety) produced by inserting the epsps gene encoding the enzyme 5-enolypyruvylshikimate-3-phosphate synthase (EPSPS) from the CP4 strain of Agrobacterium tumefaciens and glyphosate oxidase (gox) from Ochrobactrum anthropi. The herbicide-tolerant canola line GT73 (synonym RT73) was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. In order to obtain field tolerance to glyphosate herbicide, two genes, CP4 epsps and goxv247, were introduced into Brassica napus cv. Westar by Agrobacterium-mediated transformation using DNA derived from the transformation plasmid PV-BNGT04 (co-cultivation). The epsps gene codes for the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that is present in all plants, bacteria and fungi. The epsps gene put into GT73 was isolated from strain CP4 of the common soil bacterium Agrobacterium tumefaciens and is a glyphosate tolerant form of EPSPS. The EPSPS enzyme is part of an important biochemical pathway in plants called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional canola plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources. The canola line GT73 contains a second gene that codes for a modified version of glyphosate oxidase (GOX) enzyme that is ubiquitous in nature. The goxv247 gene inserted into GT73 was isolated from strain LBAA of the bacterium Ochrobactrum anthropi. Glyphosate oxidase (GOX) enzyme accelerates the normal breakdown of the herbicide glyphosate into two non-toxic compounds, aminomethylphosphonic acid (AMPA) and glyoxylate. AMPA is the principal breakdown product of glyphosate and is degraded by several microorganisms, while glyoxylate is commonly found in plant cells and is broken down by the glyoxylic pathway for lipid metabolism.
15330	MON-ØØ1Ø1-8	J101 J101	Medicago sativa - Alfalfa, Lucerne	Alfalfa with tolerance to glyphosate through introduction of the epsps gene from Agrobacterium tumefaciens strain CP4.
16135	MON-ØØ1Ø1-8 x MON-ØØ163-7	J101 x J163 J101 x J163	Medicago sativa - Alfalfa, Lucerne	A stacked line of alfalfa with tolerance to glyphosate through introduction of epsps genes from Agrobacterium tumefaciens strain CP4.
15331	MON-ØØ163-7	J163 J163	Medicago sativa - Alfalfa, Lucerne	Alfalfa with tolerance to glyphosate through introduction of the epsps gene from Agrobacterium tumefaciens strain CP4.
15332	MON-ØØ163-7 x MON-ØØ1Ø1-8	J163 x J101 J163	Medicago sativa - Alfalfa, Lucerne	A stacked herbicide-tolerant alfalfa derived from conventional cross-breeding of MON-ØØ163-7 and MON-ØØ1Ø1-8. Tolerance to glyphosate is provided through introduction of the epsps gene from Agrobacterium tumefaciens strain CP4 in both parents.
14775	MON-ØØ531-6	Bollgard ™ insect protected cotton 531	Gossypium - Cotton	Insect-resistant cotton produced by inserting the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki. The neomycin phosphotransferase II (npt II) gene was introduced as a selectable marker.
14883	MON-ØØ531-6 x MON-Ø1445-2	Roundup Ready™ Bollgard™ Cotton 1445 x 531 (MON531 X MON1445)	Gossypium - Cotton	A stacked insect-resistant and glyphosate-tolerant cotton derived from conventional cross-breeding of MON-ØØ531-6 and MON-Ø1445-2. Tolerance to the herbicide glyphosate produced through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (epsps), that confers tolerance to the herbicide glyphosate and resistance to lepidoptera insects from the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.
14776	MON-ØØ6Ø3-6	NK603 Roundup Ready™ Maize NK603	Zea mays - Maize, Corn	Maize tolerant to the herbicide glyphosate, produced through introduction of a modified (epsps) gene encoding 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS), an enzyme involved in the shikimate biochemical pathway for the production of the aromatic amino acids.
14885	MON-ØØ6Ø3-6 x MON-ØØ81Ø-6	Roundup Ready™ YieldGard™ Maize Conventional cross between LMOs NK603 and MON810	Zea mays - Maize, Corn	A stacked insect-resistant and glyphosate-tolerant corn derived from conventional cross-breeding of MON-ØØ6Ø3-6 and MON-ØØ81Ø-6. Tolerance to the herbicide glyphosate produced through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (epsps), that confers tolerance to the herbicide glyphosate and resistance to lepidoptera insects from the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki.
14777	MON-ØØ757-7	Bollgard™ insect -resistant Cotton 757	Gossypium - Cotton	Insect-resistant cotton produced by inserting the cry1Ac gene from Bacillus thuringiensis subsp. kurstaki which confers resistance to attack by the European corn borer (ECB). The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.
14750	MON-ØØ81Ø-6	YieldGard™ Maize MON810	Zea mays - Maize, Corn